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2098 cvcl 1697 u2os atcc cat  (ATCC)


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    Structured Review

    ATCC 2098 cvcl 1697 u2os atcc cat
    2098 Cvcl 1697 U2os Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2098+cvcl+1697+u2os+atcc+cat/SJSA-1/pmc11970323__mmc10-222-196-199
    Average 96 stars, based on 357 article reviews
    2098 cvcl 1697 u2os atcc cat - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    In Vitro:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    CCK-8 Assay:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    Cell Isolation:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    RNA Sequencing:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    Gene Expression:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    Sequencing:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    Methylation:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    In Vivo:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS

    Software:

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 Article ll OPEN ACCESS

    Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Cell-Light EdU Apollo567 In Vitro Image Kit Ribobio Cat#C10310 Cell Counting Kit-8 Dojindo Cat#CK04 EasySepTM Mouse Pan-Naı̈ve T cell Isolation Kit Stemcell Cat#19848a Pierce BCA Protein Quantification Kit Thermo Fisher Scientific Cat#23325 Deposited data Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE152048 Single-cell RNA-seq of Osteosarcoma Gene Expression Omnibus GEO: GSE162454 Transcriptomic of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - Genome Sequence Archive for Human GSA: HRA003260 Methylation Profiling by Array of Shanghai General Hospital Osteosarcoma Cohort National Genomics Data Center - OMIX OMIX: OMIX002042 Transcriptomic of Osteosarcoma Gene Expression Omnibus GEO: GSE42352 GDC TARGET-OS National Cancer Institute TARGET’s Study of Osteosarcoma - NCI (cancer.gov) Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX: OMIX007614 Bulk RNA-seq of DuNN Mouse Models in vivo National Genomics Data Center - Genome Sequence Archive (This study) GSA: CRA021534 Bulk RNA-seq of 143B in vitro National Genomics Data Center - Genome Sequence Archive for Human (This study) GSA: HRA009828 Mouse Gut Fecal Metabolomics MetaboLights (This study) MetaboLights: MTBLS11985 Experimental models: Cell lines HOS ATCC Cat#CRL-1543 CVCL_0312 HOS/MNNG ATCC Cat#CRL-1547 CVCL_0439 Cl#5 143B ATCC Cat#CRL-8303 CVCL_2270 SJSA-1 ATCC Cat#CRL-2098 CVCL_1697 U2OS ATCC Cat#HTB-96 CVCL_0042 DuNN Dunn Lab CVCL_W629 HCT116-MTAP-KO-11B1 Kyinno Bio Cat#KC-1405 HEK-293T ATCC Cat#CRL-3216 CVCL_0063 Human Mesenchymal Stem Cell (hMSC) Cell line This study N/A Experimental models: Organisms/strains C3H/HeNCrl Charlers River RRID: IMSR_CRL:025 BALB/cJGpt-Foxn1nu/Gpt Charlers River RRID: IMSR_GPT: D000521 Oligonucleotides See Table S8 for oligonucleotides This study N/A Software and algorithms GraphPad Prism (version 8) GraphPad RRID:SCR_002798 RStudio posit RRID:SCR_000432 ImageJ ImageJ RRID:SCR_003070 R software (version 4.2.0) The R Foundation RRID:SCR_001905 FlowJo (version 10) FlowJo RRID:SCR_008520 Leica Application Suite X (LAS X) Leica RRID:SCR_013673 GRCh38 genome NIH Homo sapiens genome assembly GRCh38 - NCBI - NLM (nih.gov) mm10 genome NIH Mus musculus genome assembly GRCm38 - NCBI - NLM (nih.gov) (Continued on next page) e3 Cell Reports Medicine 6, 101977, March 18, 2025 OPEN ACCESS



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